The CERS4 Polyclonal Antibody (PAC019927) is a valuable tool for research involving CERS4, a key enzyme involved in ceramide synthesis. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blotting. By binding specifically to the CERS4 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in lipid metabolism and cell signaling pathways.CERS4 is a crucial enzyme in the sphingolipid metabolism pathway, playing a significant role in the regulation of cellular processes such as apoptosis, proliferation, and cell differentiation.
Dysregulation of ceramide synthesis, in which CERS4 is involved, has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic disorders. Investigating the function of CERS4 and its role in lipid metabolism can provide insights into the pathogenesis of these conditions and potentially lead to the development of targeted therapies.
Antibody Name:
CERS4 Antibody (PACO19927)
Antibody SKU:
PACO19927
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human CERS4
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO19927(CERS4 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO19927(CERS4 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The LASS (longevity assurance homolog) family members are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1, LASS2, LASS3, LASS4, LASS5 and LASS6) and they are all involved in sphingolipid synthesis. LASS4 is a 394 amino acid, endoplasmic reticulum, multi-pass membrane protein. LASS4 increases the levels of long ceramides such as C22:0- and C24:0-ceramides. In cells deficient for CLN9, as observed in neuronal ceroid lipofuscinosis (NCL) or Batten disease, LASS4 can increase ceramide levels and partially correct growth and apoptosis.
Synonyms:
ceramide synthase 4
UniProt Protein Function:
Lass4: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.Protein type: Membrane protein, multi-pass; Membrane protein, integral; DNA-bindingChromosomal Location of Human Ortholog: 19p13.2Cellular Component: endoplasmic reticulum; endoplasmic reticulum membraneMolecular Function: sphingosine N-acyltransferase activityBiological Process: ceramide biosynthetic process; sphingolipid biosynthetic process
