The CHEK2 Polyclonal Antibody (CAB2145) is a valuable tool for researchers studying the CHEK2 gene, which plays a crucial role in DNA damage response and cell cycle regulation. This antibody, raised in rabbits, specifically targets the CHEK2 protein and is highly reactive with human samples, making it an ideal choice for Western blot applications.CHEK2, a key player in DNA repair pathways, is known for its role in maintaining genomic stability and preventing the development of cancer. Mutations in the CHEK2 gene have been linked to an increased risk of certain types of cancer, making it a promising target for cancer research and personalized medicine approaches.
By using the CHEK2 Polyclonal Antibody, researchers can effectively study the expression and function of CHEK2 in various cellular contexts, providing valuable insights into its role in cancer development and potential therapeutic interventions. This antibody is a valuable tool for advancing our understanding of DNA damage response mechanisms and identifying new strategies for cancer treatment.
Product Name:
Chk2 Rabbit Polyclonal Antibody
SKU:
CAB2145
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Chk2 (NP_009125.1).
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
11200
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Chk2 Rabbit pAb (CAB2145) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
