The CHGA Polyclonal Antibody (PAC020911) is a valuable tool for research into chromogranin A (CHGA), a protein involved in neuroendocrine cell function and hormone regulation. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.CHGA is a key player in the regulation of catecholamine secretion and is commonly used as a marker for neuroendocrine tumors. Its expression has also been linked to various physiological and pathological conditions, making it a target of interest in research on cancer, cardiovascular disease, and neurodegenerative disorders.
By detecting and analyzing CHGA protein levels, researchers can gain insights into the role of neuroendocrine cells in health and disease.Overall, the CHGA Polyclonal Antibody (PAC020911) provides a reliable tool for investigating the functions and mechanisms of CHGA in different biological contexts, offering valuable data for studies in endocrinology, oncology, and neuroscience.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20911(CHGA Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 8%SDS-PAGE,Lysate: 40 μgPrimary antibody: PACO20911(CHGA Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 10 minutes.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20911(CHGA Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene.
Synonyms:
chromogranin A (parathyroid secretory protein 1)
UniProt Protein Function:
CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.
UniProt Protein Details:
Protein type:Secreted, signal peptide; Secreted; Vesicle; Cell adhesion
Chromosomal Location of Human Ortholog: 14q32
Cellular Component: extracellular space; perinuclear region of cytoplasm; secretory granule
Biological Process: defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; mast cell activation; mast cell chemotaxis; mast cell cytokine production; mast cell degranulation; negative regulation of catecholamine secretion; negative regulation of vasodilation; organelle organization and biogenesis; positive regulation of cAMP metabolic process; regulation of blood pressure; regulation of the force of heart contraction
NCBI Summary:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
