The CHPF Polyclonal Antibody (PAC023799) is a valuable tool for researchers studying the CHPF protein, which plays a key role in various cellular processes. This antibody, produced using rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By binding to the CHPF protein, researchers can accurately detect and analyze its expression in different cell types, making it an essential component for studies in cell biology and cancer research.
CHPF, also known as chondrocyte protein with a polyproline II helix motif, is involved in important cellular functions such as cell growth, differentiation, and apoptosis. Its involvement in these processes highlights its potential significance in diseases like cancer, where dysregulation of cell growth and apoptosis pathways can lead to tumorigenesis. Understanding the role of CHPF is crucial for developing targeted therapies that can manipulate these pathways for therapeutic benefit.
Synthesized peptide derived from internal of human CHSS2.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from LOVO cells and K562 cells, using CHSS2 antibody.
Immunofluorescence analysis of MCF-7 cells, using CHSS2 antibody.
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using CHSS2 antibody.
Background:
Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Isoform 2 may facilitate PARK2 transport into the mitochondria. In collaboration with PARK2, isoform 2 may enhance cell viability and protect cells from oxidative stress.
Synonyms:
CHSS2; DKFZP434E0423; EC 2.4.1.175; EC 2.4.1.226;
UniProt Protein Function:
CHPF: a type II transmembrane protein. May act as a specific activating factor for chondroitin synthase in chondroitin biosynthesis and polymerization. Protein type: Glycan Metabolism - chondroitin sulfate biosynthesis; EC 2.4.1.226; EC 2.4.1.175; Membrane protein, integral; TransferaseChromosomal Location of Human Ortholog: 2q35Cellular Component: Golgi membraneMolecular Function: glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activityBiological Process: chondroitin sulfate biosynthetic process
