The CHST9 Polyclonal Antibody (PAC053478) is a versatile tool for researchers studying CHST9, a critical enzyme involved in glycosaminoglycan biosynthesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the CHST9 protein, this antibody enables the detection and analysis of CHST9 in various cell types, making it an essential component for investigations in glycobiology and cancer research.CHST9, also known as carbohydrate sulfotransferase 9, plays a crucial role in modifying proteoglycans, which are involved in cell signaling, cell adhesion, and extracellular matrix organization.
Dysregulation of CHST9 has been implicated in various diseases, including cancer and inflammatory disorders. Therefore, studying the function of CHST9 is essential for understanding its role in disease progression and developing targeted therapies for these conditions.Researchers conducting studies on glycosaminoglycan biosynthesis, extracellular matrix remodeling, and cell signaling pathways will find the CHST9 Polyclonal Antibody to be a valuable asset in their experimental endeavors. Its specificity and sensitivity make it an ideal reagent for exploring the intricate mechanisms of CHST9 function in physiological and pathological contexts.
Western Blot. Positive WB detected in: Mouse brain tissue, Mouse liver tissue. All lanes: CHST9 antibody at 2.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 kDa. Observed band size: 53 kDa.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO53478 at dilution of 1:100.
Background:
Catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Participates in biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Has a higher activity toward carbonic anhydrase VI than toward lutropin. Only active against terminal GalNAcbeta1,GalNAcbeta. Isoform 2, but not isoform 1, is active toward chondroitin.
CHST9: Catalyzes the transfer of sulfate to position 4 of non- reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Participates in biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Has a higher activity toward carbonic anhydrase VI than toward lutropin. Only active against terminal GalNAcbeta1,GalNAcbeta. Isoform 2, but not isoform 1, is active toward chondroitin. Belongs to the sulfotransferase 2 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Transferase; Membrane protein, integral; EC 2.8.2.-Chromosomal Location of Human Ortholog: 18q11.2Cellular Component: Golgi membraneMolecular Function: chondroitin 4-sulfotransferase activity; N-acetylgalactosamine 4-O-sulfotransferase activityBiological Process: chondroitin sulfate biosynthetic process; hormone biosynthetic process; proteoglycan biosynthetic process; sulfur metabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Sulfate groups on carbohydrates confer highly specific functions to glycoproteins, glycolipids, and proteoglycans, and are critical for cell-cell interaction, signal transduction, and embryonic development. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2011]
