The CIRBP Monoclonal Antibody (CAB19290) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the Cirbp protein, it enables accurate detection and analysis in different cell types, making it ideal for studies in molecular biology and cellular physiology.
This antibody is validated for use in WB, IHC-P, IP, ELISA, IF-P applications and has demonstrated reactivity against Human samples.
Product Name:
CIRBP Monoclonal Antibody
SKU:
CAB19290
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC2472
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cell
IF-P
1:50 - 1:200
IHC-P
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CIRP, CIRBP
Positive Sample:
293T, HepG2, SH-SY5Y
Cellular Localization:
Cytoplasm, Nucleus, Nucleoplasm.
Calculated MW:
19kDa
Observed MW:
19kDa
Enables mRNA 3'-UTR binding activity and small ribosomal subunit rRNA binding activity. Involved in mRNA stabilization; positive regulation of translation; and response to UV. Located in cytoplasm and nucleoplasm.
Purification Method
Affinity purification
Gene ID
1153
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CIRBP Rabbit mAb (CAB19290) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using CIRBP Rabbit mAb (CAB19290) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using CIRBP Rabbit mAb (CAB19290) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human breast cancer tissue using CIRBP Rabbit mAb (CAB19290, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
