The CKAP4 Antibody (CAB7777) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the CKAP4 protein, this antibody enables precise detection and analysis in a wide range of cell types, making it an ideal choice for studies in cell biology and cancer research.CKAP4, also known as cytoskeleton-associated protein 4, plays a crucial role in cell signaling, proliferation, and migration, making it an important target for understanding cellular function and dysfunction.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CKAP4 Antibody
SKU:
CAB7777
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p63, CLIMP63, CLIMP-63, ERGIC-63, CKAP4
Positive Sample:
293T, HepG2, MCF7, NCI-H460, Mouse testis
Cellular Localization:
Cell Membrane, Cytoplasm, Endoplasmic Reticulum Membrane, Single-Pass Type Ii Membrane Protein, Cytoskeleton, Perinuclear Region.
Calculated MW:
66kDa
Observed MW:
66kDa
Enables RNA binding activity. Located in several cellular components, including lipid droplet; nuclear speck; and rough endoplasmic reticulum.
Purification Method
Affinity purification
Gene ID
10970
RRID
AB_2768932
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CKAP4 Rabbit pAb (CAB7777) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal immunofluorescence analysis of U2OS cells using CKAP4 Rabbit pAb (CAB7777) at dilution of 1:100. Blue: DAPI for nuclear staining.
