The Casein Kinase 2 alpha (CSNK2A1) Monoclonal Antibody (CAB19683) is a high-quality antibody developed for reliable detection and analysis of target proteins. Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. RRID AB_2862732 Gene ID 1457 Swiss Prot Synonym CKII; Cka1; Cka2; CK2A1; OCNDS; [KD Validated] Casein Kinase 2 alpha (CSNK2A1)
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. RRID AB_2862732 Gene ID 1457 Swiss Prot Synonym CKII; Cka1; Cka2; CK2A1; OCNDS; [KD Validated] Casein Kinase 2 alpha (CSNK2A1)
Purification Method:
Affinity purification
Gene ID:
1457
RRID:
AB_2862732
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using [KD Validated] Casein Kinase 2 alpha (CSNK2A1) Rabbit mAb (CAB19683) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from wild type(WT) and Casein Kinase 2 alpha (CSNK2A1) knockdown (KD) 293T cells, using [KD Validated] Casein Kinase 2 alpha (CSNK2A1) Rabbit mAb (CAB19683) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
