Integrin alpha V (ITGAV/CD51) Monoclonal Antibody (CAB19071)
The Integrin alpha V (ITGAV/CD51) Monoclonal Antibody (CAB19071) is a high-quality antibody developed for reliable detection and analysis of target proteins. The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
This antibody is validated for use in WB, IHC-P, ELISA, FC (intra) applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Integrin alpha V (ITGAV/CD51) Monoclonal Antibody
SKU:
CAB19071
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC50621
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAFC (intra)
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:1000 - 1:4000
FC (intra)
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD51, MSK8, VNRA, VTNR, Integrin alpha V (ITGAV/CD51)
Positive Sample:
A549, MCF7, NIH/3T3, C6, Mouse brain, Mouse kidney, Rat kidney, Rat lung
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
116kDa
Observed MW:
140kDa
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Purification Method
Affinity purification
Gene ID
3685
RRID
AB_2862563
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Integrin alpha V (ITGAV/CD51) Rabbit mAb (CAB19071) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Integrin alpha V (ITGAV/CD51) Rabbit mAb (CAB19071) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Integrin alpha V (ITGAV/CD51) Rabbit mAb (CAB19071) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Integrin alpha V (ITGAV/CD51) Rabbit mAb (CAB19071) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Integrin alpha V (ITGAV/CD51) Rabbit mAb (CAB19071) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Integrin alpha V (ITGAV/CD51) Rabbit mAb (CAB19071) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Flow cytometry:1X10^6 Daudi cells (negative control,left) and HUVEC cells (right) were intracellularly-stained with Integrin alpha V (ITGAV/CD51) Rabbit mAb(CAB19071, 2.5 μg/mL,orange line) or Rabbit IgG isotype control (AC042, 2.5 μg/mL,blue line),followed by FITC conjugated goat anti-rabbit pAb(1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Daudi cells cells (negative control,left) and BEWO cells (right) were intracellularly-stained with Integrin alpha V (ITGAV/CD51) Rabbit mAb(CAB19071, 2.5 μg/mL,orange line) or Rabbit IgG isotype control (AC042, 2.5 μg/mL,blue line),followed by FITC conjugated goat anti-rabbit pAb(1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Daudi cells (negative control,left) and U-251MG cells (right) were intracellularly-stained with Integrin alpha V (ITGAV/CD51) Rabbit mAb(CAB19071, 2.5 μg/mL,orange line) or Rabbit IgG isotype control (AC042, 2.5 μg/mL,blue line),followed by FITC conjugated goat anti-rabbit pAb(1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
