The CLPS Antibody (CAB12772) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been validated for use in Western blot applications, ensuring accurate detection and analysis of CLPS in various cellular samples.CLPS, also known as Colipase, is an essential cofactor for pancreatic lipase, facilitating the hydrolysis of dietary triglycerides in the small intestine. Dysfunction or dysregulation of CLPS can lead to impaired digestion and absorption of fats, resulting in metabolic disorders such as obesity and hyperlipidemia. By targeting CLPS with this antibody, researchers can gain insights into the molecular mechanisms underlying lipid metabolism and potentially develop novel therapeutic strategies for addressing related diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
CLPS Antibody
SKU:
CAB12772
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CLPS, colipase
Positive Sample:
Mouse pancreas, Rat pancreas
Cellular Localization:
Secreted.
Calculated MW:
12kDa
Observed MW:
12kDa
The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1208
RRID
AB_2759616
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CLPS Rabbit pAb (CAB12772) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
