Anti-CLPS Antibody (CAB6364)

Antibody Name:	Anti-CLPS Antibody
Antibody SKU:	CAB6364
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-112 of human CLPS (NP_001823.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-112 of human CLPS (NP_001823.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	APGP RGII INLE NGEL CMNS AQCK SNCC QHSS ALGL ARCT SMAS ENSE CSVK TLYG IYYK CPCE RGLT CEGD KTIV GSIT NTNF GICH DAGR SKQ
Gene ID:	1208
Uniprot:	P04118
Cellular Location:	Secreted
Calculated MW:	11kDa
Observed MW:	12kDa

Synonyms:

CLPS, colipase

Background:

The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CLPS: Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Belongs to the colipase family.
UniProt Protein Details:	Protein type:Activator; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 6p21.31 Cellular Component: extracellular region Molecular Function:enzyme activator activity Biological Process: positive regulation of catalytic activity; phototransduction, visible light; lipid metabolic process; retinoid metabolic process; lipid catabolic process; lipid digestion
NCBI Summary:	The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:	P04118
NCBI GenInfo Identifier:	116900
NCBI Gene ID:	1208
NCBI Accession:	P04118.2
UniProt Secondary Accession:	P04118,Q5T9G7, Q5U809,
UniProt Related Accession:	P04118
Molecular Weight:	112
NCBI Full Name:	Colipase
NCBI Synonym Full Names:	colipase, pancreatic
NCBI Official Symbol:	CLPS
NCBI Protein Information:	colipase; pancreatic colipase preproprotein
UniProt Protein Name:	Colipase
Protein Family:	Colipase
UniProt Gene Name:	CLPS
UniProt Entry Name:	COL_HUMAN

	Western blot analysis of extracts of mouse pancreas, using CLPS antibody (CAB6364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using CLPS antibody (CAB6364). Blue: DAPI for nuclear staining.

Antibodies

Anti-CLPS Antibody (CAB12772)

Anti-CLPS Antibody (CAB13480)

CLPS Antibody (PACO08470)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)