The CNPY3 Antibody (CAB7176) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that binds members of the toll-like receptor protein family and functions as a chaperone to aid in folding and export of these proteins. Alternative splicing results in multiple transcript variants. Naturally occuring readthrough transcription occurs between this locus and the downstream GNMT (glycine N-methyltransferase) gene and is represented with GeneID:107080644.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CNPY3 Antibody
SKU:
CAB7176
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAG4A, DEE60, ERDA5, TNRC5, EIEE60, PRAT4A, CNPY3
Positive Sample:
HepG2, MCF7, THP-1, HL-60, U-937
Cellular Localization:
Endoplasmic Reticulum.
Calculated MW:
31kDa
Observed MW:
38kDa
This gene encodes a protein that binds members of the toll-like receptor protein family and functions as a chaperone to aid in folding and export of these proteins. Alternative splicing results in multiple transcript variants. Naturally occuring readthrough transcription occurs between this locus and the downstream GNMT (glycine N-methyltransferase) gene and is represented with GeneID:107080644.
Purification Method
Affinity purification
Gene ID
10695
RRID
AB_2767726
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CNPY3 Rabbit pAb (CAB7176) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 15s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using CNPY3 Rabbit pAb (CAB7176) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
