The CES2 Antibody (CAB1514) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in a variety of research applications including Western blotting.Cocaine esterase is a key enzyme involved in the breakdown of cocaine into inactive metabolites, ultimately reducing the drug's psychoactive effects. Understanding the mechanisms and regulation of cocaine esterase activity is crucial for developing treatments for drug addiction and overdose.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CES2 Antibody
SKU:
CAB1514
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
iCE, CE-2, PCE-2, CES2A1, CES2
Positive Sample:
SW620, Mouse liver
Cellular Localization:
Endoplasmic Reticulum Lumen.
Calculated MW:
62kDa
Observed MW:
67kDa
This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
8824
RRID
AB_2762026
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CES2 Rabbit pAb (CAB1514) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of MCF-7 cells using CES2 Rabbit pAb (CAB1514). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
