The Collagen XVII/COL17A1 Monoclonal Antibody (CAB4808) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. RRID AB_2863351 Gene ID 1308 Swiss Prot Synonym ERED; JEB4; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2; Collagen XVII/COL17A1
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Collagen XVII/COL17A1 Monoclonal Antibody
SKU:
CAB4808
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0233
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ERED, JEB4, BP180, BPA-2, BPAG2, LAD-1, BA16H23.2, Collagen XVII/COL17A1
Positive Sample:
A431
Cellular Localization:
Cell Junction, Membrane, Secreted, Single-Pass Type Ii Membrane Protein, Basement Membrane, Extracellular Matrix, Extracellular Space, Hemidesmosome.
Calculated MW:
150kDa
Observed MW:
150-180kDa
This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. RRID AB_2863351 Gene ID 1308 Swiss Prot Synonym ERED; JEB4; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2; Collagen XVII/COL17A1
Purification Method:
Affinity purification
Gene ID:
1308
RRID:
AB_2863351
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Collagen XVII/COL17A1 Rabbit mAb (CAB4808) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Collagen XVII/COL17A1 Rabbit mAb (CAB4808) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
