The Collagen IV Monoclonal Antibody (CAB24008) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. RRID Gene ID 1286 1288 1284 1282 Swiss Prot Synonym BFH; ATS2; BFH1; CA44; Collagen IV
This antibody is validated for use in WB, ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Collagen IV Monoclonal Antibody
SKU:
CAB24008
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC62585
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISADB
Recommended Dilution:
WB
1:2000 - 1:8000
DB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BFH, ATS2, BFH1, CA44, Collagen IV
Positive Sample:
HeLa, Rat testis, Rat lung, Mouse lung
Cellular Localization:
Secreted.
Calculated MW:
164 kDa
Observed MW:
164 kDa
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. RRID Gene ID 1286 1288 1284 1282 Swiss Prot Synonym BFH; ATS2; BFH1; CA44; Collagen IV
Purification Method:
Affinity purification
Gene ID:
1286 1288 1284 1282
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells using Collagen IV Rabbit mAb (CAB24008) at 1:7000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
Western blot analysis of various lysates using Collagen IV Rabbit mAb (CAB24008) at 1:7000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
Dot-blot analysis of all sorts of peptides using Collagen IV Rabbit mAb antibody (CAB24008) at 1:1000 dilution.
