The CPS1 Monoclonal Antibody (CAB4214) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, is highly specific to CPS1 and is suitable for various applications such as Western blot, immunofluorescence, and immunohistochemistry.CPS1 plays a crucial role in the detoxification of ammonia in the body by converting it into urea. Dysregulation of CPS1 activity is associated with hyperammonemia, a condition characterized by elevated levels of ammonia in the blood.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human samples.
Product Name:
CPS1 Monoclonal Antibody
SKU:
CAB4214
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0929
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PHN, GATD6, CPSASE1, CPS1
Positive Sample:
HeLa
Cellular Localization:
Mitochondrion, Nucleus, Nucleolus, Cell Membrane.
Calculated MW:
165kDa
Observed MW:
165kDa
The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.
Purification Method
Affinity purification
Gene ID
1373
RRID
AB_2863210
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CPS1 Rabbit mAb (CAB4214) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human liver using CPS1 Rabbit mAb (CAB4214) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Confocal imaging of paraffin-embedded Human liver using CPS1 Rabbit mAb (CAB4214, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
