The CPSF73 Monoclonal Antibody (CAB2222) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human CPSF73 and has been validated for use in Western blot applications. By binding to CPSF73, this antibody allows for precise detection and analysis of the protein in various cell types, making it an essential component in studies related to gene expression regulation and RNA processing.CPSF73 is a crucial component of the cleavage and polyadenylation complex, playing a vital role in mRNA maturation. Dysregulation of CPSF73 expression or activity has been linked to various diseases, including cancer and neurological disorders.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CPSF73 Monoclonal Antibody
SKU:
CAB2222
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2567
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing.
Purification Method
Affinity purification
Gene ID
51692
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CPSF73 Rabbit mAb (CAB2222) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of lysates from rat spleen, using CPSF73 Rabbit mAb (CAB2222) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
