The CRYAA Monoclonal Antibody (CAB5111) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting CRYAA in various biological samples.CRYAA, also known as α-crystallin A, is a small heat shock protein with chaperone-like activity, important for maintaining the transparency and refractive index of the lens. Dysregulation of CRYAA has been linked to cataract formation and other eye diseases, making it a target of interest in ophthalmology research.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
CRYAA Monoclonal Antibody
SKU:
CAB5111
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC1245
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CRYA1, HSPB4, CTRCT9, CRYAA
Positive Sample:
Mouse eye, Rat eye
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
20kDa
Observed MW:
18kDa
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
Purification Method
Affinity purification
Gene ID
1409
RRID
AB_2863449
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CRYAA Rabbit mAb (CAB5111) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of paraffin-embedded rat eye using CRYAA Rabbit mAb (CAB5111) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
