The Cullin-4B Polyclonal Antibody (CAB5400) is a vital tool for researchers studying the Cullin-4B protein, a key player in the ubiquitin-proteasome system. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot analyses. By targeting the Cullin-4B protein, researchers can gain insights into its functions and roles in various cellular processes, particularly in protein degradation pathways.Cullin-4B is a crucial component of the Cullin-RING E3 ubiquitin ligase complex, playing a fundamental role in the targeted degradation of proteins involved in cell cycle regulation, DNA damage response, and signal transduction pathways.
Dysregulation of Cullin-4B has been linked to various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic interventions. By using the Cullin-4B Polyclonal Antibody, researchers can unravel the intricate mechanisms underlying Cullin-4B-mediated protein degradation and its implications in disease pathogenesis.
Product Name:
CUL4B Rabbit Polyclonal Antibody
SKU:
CAB5400
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CUL4B (NP_003579.3).
This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
8450
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CUL4B Rabbit pAb (CAB5400) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
