The Cyclin E2 Monoclonal Antibody (CAB9305) is a high-quality antibody developed for reliable detection and analysis of target proteins. Cyclin E2 is a key regulatory protein involved in the progression of the cell cycle, specifically in the G1/S transition. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting Cyclin E2 in human samples through Western blot analysis.Cyclin E2 is known to interact with cyclin-dependent kinases and play a crucial role in cell division and proliferation. Dysregulation of Cyclin E2 expression has been implicated in various types of cancer, making it a promising target for cancer therapy development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Cyclin E2 Monoclonal Antibody
SKU:
CAB9305
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1515
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CYCE2, Cyclin E2
Positive Sample:
MCF7, Jurkat, SH-SY5Y
Cellular Localization:
Nucleus.
Calculated MW:
47kDa
Observed MW:
47kDa
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells.
Purification Method
Affinity purification
Gene ID
9134
RRID
AB_2863707
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cyclin E2 Rabbit mAb (CAB9305) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
