The CYFIP1 Antibody (CAB10291) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that regulates cytoskeletal dynamics and protein translation. The encoded protein is a component of the WAVE regulatory complex (WRC), which promotes actin polymerization. This protein also interacts with the synaptic functional regulator FMR1 protein and translation initiation factor 4E to inhibit protein translation. A large chromosomal deletion including this gene is associated with increased risk of schizophrenia and epilepsy in human patients. Reduced expression of this gene has been observed in various human cancers and the encoded protein may inhibit tumor invasion.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CYFIP1 Antibody
SKU:
CAB10291
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SHYC, SRA1, SRA-1, P140SRA-1, CYFIP1
Positive Sample:
B cells, BT-474, HepG2, HT-1080, Mouse lung, Mouse brain
This gene encodes a protein that regulates cytoskeletal dynamics and protein translation. The encoded protein is a component of the WAVE regulatory complex (WRC), which promotes actin polymerization. This protein also interacts with the synaptic functional regulator FMR1 protein and translation initiation factor 4E to inhibit protein translation. A large chromosomal deletion including this gene is associated with increased risk of schizophrenia and epilepsy in human patients. Reduced expression of this gene has been observed in various human cancers and the encoded protein may inhibit tumor invasion.
Purification Method
Affinity purification
Gene ID
23191
RRID
AB_2757834
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CYFIP1 Rabbit pAb (CAB10291) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of BT-474 cells using 3 μg CYFIP1 antibody (CAB10291). Western blot was performed from the immunoprecipitate using CYFIP1 antibody (CAB10291) at a dilution of 1:500.
