The CYP1A1/CYP1A2 Antibody (PAC022041) is a highly specific tool for researchers studying the cytochrome P450 enzymes CYP1A1 and CYP1A2. These enzymes play a crucial role in the metabolism of exogenous and endogenous compounds, including drugs and environmental toxins. This polyclonal antibody is raised in rabbits and has been validated for use in various applications, including Western blotting.CYP1A1 and CYP1A2 are known to be inducible by environmental pollutants and are involved in the activation and detoxification of carcinogens.
Understanding the expression and activity of these enzymes is important for research in pharmacology, toxicology, and personalized medicine. The CYP1A1/CYP1A2 antibody enables precise detection and analysis of these enzymes, providing valuable insights into their function in health and disease.
Antibody Name:
CYP1A1/CYP1A2 Antibody (PACO22041)
Antibody SKU:
PACO22041
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human Cytochrome P450 1A1/2.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COS-7 cells, RAW264.7 cells and HT-29 cells, using Cytochrome P450 1A1/2 antibody.
Background:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Synonyms:
AHH; AHRR; CP11; CYP1; CYPIA1
UniProt Protein Function:
CYP1A1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.
UniProt Protein Details:
Protein type:Cofactor and Vitamin Metabolism - retinol; Amino Acid Metabolism - tryptophan; Oxidoreductase; EC 1.14.14.1; Xenobiotic Metabolism - metabolism by cytochrome P450
Molecular Function:oxidoreductase activity, acting on diphenols and related substances as donors; flavonoid 3'-monooxygenase activity; enzyme binding; iron ion binding; heme binding; oxidoreductase activity; steroid hydroxylase activity; oxygen binding; demethylase activity
Biological Process: coumarin metabolic process; response to nematode; response to arsenic; response to lipopolysaccharide; cellular lipid metabolic process; response to vitamin A; response to antibiotic; embryonic development ending in birth or egg hatching; flavonoid metabolic process; porphyrin metabolic process; response to wounding; arachidonic acid metabolic process; drug metabolic process; aging; response to food; response to drug; insecticide metabolic process; camera-type eye development; response to iron(III) ion; response to virus; epoxygenase P450 pathway; response to herbicide; dibenzo-p-dioxin catabolic process; vitamin D metabolic process; cell proliferation; response to hyperoxia; gut development; xenobiotic metabolic process; maternal process involved in parturition; response to hypoxia; 9-cis-retinoic acid biosynthetic process; amine metabolic process; hydrogen peroxide biosynthetic process
NCBI Summary:
This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq, Jul 2008]
