The CYP51A1 Polyclonal Antibody (CAB6252) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity specifically with human samples and is optimized for use in Western blot applications. By binding to the CYP51A1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an excellent choice for studies in the fields of biochemistry and pharmacology.CYP51A1, also known as lanosterol 14-alpha demethylase, is a key enzyme in the cholesterol biosynthesis pathway, making it a potential target for therapeutic interventions in diseases related to cholesterol metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CYP51A1 Polyclonal Antibody
SKU:
CAB6252
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1595
RRID
AB_2766859
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CYP51A1 Rabbit pAb (CAB6252) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
