The DAO Monoclonal Antibody (CAB22081) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced using hybridoma technology, offers superior specificity and sensitivity for detecting DAO in various biological samples.DAO is an essential enzyme involved in the catabolism of D-amino acids, which are increasingly recognized as important modulators of neurotransmission and immune response. Dysregulation of DAO activity has been linked to various neurological disorders, including schizophrenia and Alzheimer's disease, as well as inflammatory conditions such as irritable bowel syndrome.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DAO Monoclonal Antibody
SKU:
CAB22081
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC54988
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAAO, OXDA, DAMOX, DAO
Positive Sample:
HepG2, A-549, Mouse brain, Rat brain
Cellular Localization:
Peroxisome.
Calculated MW:
39kDa
Observed MW:
39kDa
This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.
Purification Method
Affinity purification
Gene ID
1610
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DAO Rabbit mAb (CAB22081) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of various lysates using DAO Rabbit mAb (CAB22081) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
