The DDAH1 Monoclonal Antibody (CAB4591) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
DDAH1 Monoclonal Antibody
SKU:
CAB4591
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
ARC1043
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DDAH, DDAHI, DDAH-1, HEL-S-16, DDAH1
Positive Sample:
293T, HepG2, U-251MG, Rat kidney
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
31kDa
Observed MW:
31-37kDa
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
Purification Method
Affinity purification
Gene ID
23576
RRID
AB_2863304
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DDAH1 Rabbit mAb (CAB4591) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
Western blot analysis of lysates from Rat kidney using DDAH1 Rabbit mAb (CAB4591) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180 s.
