The DDAH2 Antibody (CAB6457) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications. By targeting the DDAH2 protein, this antibody enables precise detection and analysis in a variety of cell types, facilitating research in cardiovascular disease, hypertension, and other conditions related to nitric oxide dysregulation.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DDAH2 Antibody
SKU:
CAB6457
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
G6a, DDAH, NG30, DDAHII, HEL-S-277, DDAH2
Positive Sample:
SW620, BT-474, MCF7, Mouse lung, Rat brain
Cellular Localization:
Cytoplasm, Mitochondrion.
Calculated MW:
30kDa
Observed MW:
30kDa
This gene encodes a dimethylarginine dimethylaminohydrolase. The encoded enzyme functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
23564
RRID
AB_2767059
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DDAH2 Rabbit pAb (CAB6457) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using DDAH2 Rabbit pAb (CAB6457). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
