The DDB2 Monoclonal Antibody (CAB11615) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and sensitive for detecting DDB2 protein in human samples, making it ideal for use in Western blot assays.DDB2 is essential for the recognition and repair of DNA damage caused by various sources, including UV radiation and chemical agents. Dysregulation of DDB2 has been linked to genomic instability and increased cancer risk, making it a promising target for cancer research and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DDB2 Monoclonal Antibody
SKU:
CAB11615
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0644
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
XPE, DDBB, UV-DDB2, DDB2
Positive Sample:
HeLa, U-87MG
Cellular Localization:
Nucleus.
Calculated MW:
48kDa
Observed MW:
48kDa
This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1643
RRID
AB_2861608
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DDB2 Rabbit mAb (CAB11615) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
