The DDX5 Antibody (CAB5296) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications.The DDX5 Polyclonal Antibody specifically binds to the DDX5 protein, enabling researchers to detect and analyze its expression in various cell types. This makes it a valuable asset for studies in molecular biology, RNA biology, and cancer research, where DDX5 has been implicated in tumor progression and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DDX5 Antibody
SKU:
CAB5296
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p68, HLR1, G17P1, HUMP68, DDX5
Positive Sample:
HeLa, HT-29, Mouse testis, Mouse brain
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
69kDa
Observed MW:
68kDa
This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1655
RRID
AB_2766108
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DDX5 Rabbit pAb (CAB5296) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates using DDX5 Rabbit pAb (CAB5296) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 120s.
