The DDX50 Polyclonal Antibody (CAB8628) is a valuable tool for researchers studying DDX50, a protein involved in RNA processing and translation regulation. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding to the DDX50 protein, this antibody allows for the detection and analysis of DDX50 in various cell types, making it an essential component for research in molecular biology and cancer biology.DDX50, a member of the DEAD-box RNA helicase family, plays a crucial role in RNA metabolism and is implicated in various cellular processes such as pre-mRNA splicing, ribosome biogenesis, and translation initiation.
Dysregulation of DDX50 has been linked to cancer development, making it a promising target for therapeutic interventions. By understanding the function and regulation of DDX50, researchers can gain insights into the molecular mechanisms underlying cancer and potentially identify novel therapeutic strategies targeting this protein.
Product Name:
DDX50 Rabbit Polyclonal Antibody
SKU:
CAB8628
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1).
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.
Purification Method:
Affinity purification
Gene ID:
79009
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DDX50 Rabbit pAb (CAB8628) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
