The DFFA Antibody (CAB12431) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is suitable for use in Western blot applications and has been validated for its high reactivity with human samples.DFFA, also known as DNA fragmentation factor subunit alpha, is involved in DNA fragmentation during apoptosis, making it a vital target in cancer research and understanding cell death mechanisms. By binding to the DFFA protein, this polyclonal antibody allows for the detection and analysis of DFFA in various cell types, providing valuable insights into apoptosis regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DFFA Antibody
SKU:
CAB12431
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DFF1, ICAD, DFF-45, DFFA
Positive Sample:
Jurkat treated with Etoposide, HeLa
Cellular Localization:
Cytoplasm.
Calculated MW:
37kDa
Observed MW:
35kDa/45kDa
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1676
RRID
AB_2759273
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DFFA Rabbit pAb (CAB12431) at 1:1000 dilution. Jurkat cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
