The DGCR8 Monoclonal Antibody (CAB3977) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly specific to DGCR8, a key protein involved in microRNA maturation. DGCR8 plays a crucial role in the microRNA processing pathway, where it helps in the cleavage of precursor miRNA into mature miRNA. Dysregulation of DGCR8 has been linked to various diseases, including cancer and neurodevelopmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
DGCR8 Monoclonal Antibody
SKU:
CAB3977
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0289
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Gy1, pasha, DGCRK6, C22orf12, DGCR8
Positive Sample:
HeLa, RD, U-251MG, Mouse brain
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
86kDa
Observed MW:
100kDa
This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
54487
RRID
AB_2863167
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DGCR8 Rabbit mAb (CAB3977) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from Mouse brain, using DGCR8 Rabbit mAb (CAB3977) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
