The DGKA Antibody (CAB6896) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, offers high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the DGKA protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in biochemistry and molecular biology.DGKA is known for its involvement in diverse cellular processes, including cell proliferation, differentiation, and apoptosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DGKA Antibody
SKU:
CAB6896
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAGK, DAGK1, DGK-alpha, DGKA
Positive Sample:
HeLa, Jurkat, Molt-4
Cellular Localization:
Cytosol, Plasma Membrane.
Calculated MW:
83kDa
Observed MW:
80kDa
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
1606
RRID
AB_2767455
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DGKA Rabbit pAb (CAB6896) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
