The DHEA (Dehydroepiandrosterone) ELISA Kit is designed for the quantitative detection of Dehydroepiandrosterone (DHEA) levels in various biological samples. DHEA is an important endogenous steroid hormone involved in a variety of functions, including the production of adrenal androgens and sex steroids, as well as in the neurosteroid pathway, ultimately impacting cognition, mood, and physical health. It plays a significant role in the body's endocrine and immune systems, underpinning various physiological processes and has been implicated in age-related diseases, mood disorders, and autoimmune diseases. Accurate measurement of DHEA is essential for understanding its role and impact on health and disease as well as for developing targeted therapeutic strategies.
Assay Genie's DHEA ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's DHEA ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
DHEA (Dehydroepiandrosterone) ELISA Kit
SKU:
AEES00004
Target:
DHEA (Dehydroepiandrosterone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHEA. During the reaction, DHEA in the sample or standard competes with a fixed amount of DHEA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHEA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHEA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
96-110
94-102
Average (%)
91
102
97
1:4
Range (%)
85-98
98-107
91-98
Average (%)
90
103
94
1:8
Range (%)
93-103
96-105
84-93
Average (%)
97
100
90
1:16
Range (%)
96-109
87-99
96-107
Average (%)
101
95
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-103
100
EDTA plasma (n=5)
87-102
95
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.02
4.54
0.42
1.07
4.19
Standard deviation
0.03
0.05
0.22
0.02
0.05
0.17
C V (%)
6.52
4.9
4.85
4.76
4.67
4.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of DHEA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes DHEA in samples. No significant cross-reactivity or interference between DHEA and analogues was observed.
