The DHT (Dihydrotestosterone) ELISA Kit is specifically designed for the precise measurement of Dihydrotestosterone levels in various biological samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.Dihydrotestosterone is a potent androgen hormone that plays a crucial role in the development and maintenance of male characteristics. It is involved in various physiological processes, including the regulation of hair growth, muscle development, and sexual function.
Dihydrotestosterone has also been implicated in conditions such as male pattern baldness, prostate cancer, and hirsutism, making it an important biomarker for studying these disorders and exploring potential therapeutic interventions.With its reliable performance and comprehensive applications, the DHT ELISA Kit is a valuable tool for researchers in the fields of endocrinology, oncology, and reproductive medicine. Get accurate and reliable results with this cutting-edge assay kit from Assay Genie.
Product Name:
DHT (Dihydrotestosterone) ELISA Kit
SKU:
UNES00021
Target:
DHT (Dihydrotestosterone) (UNES00021)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
23.44 pg/mL
Detection range:
39.06-2500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHT. During the reaction, DHT in the sample or standard competes with a fixed amount of DHT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
96-111
91-103
Average (%)
94
102
98
1:4
Range (%)
88-100
97-112
93-105
Average (%)
95
104
100
1:8
Range (%)
90-103
98-110
90-104
Average (%)
96
104
95
1:16
Range (%)
84-95
91-106
93-108
Average (%)
90
97
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
120.59
263.0
1006.26
109.96
261.58
989.74
Standard deviation
8.02
14.02
32.7
6.86
14.28
30.48
C V (%)
6.65
5.33
3.25
6.24
5.46
3.08
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of DHT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes DHT in samples. No significant cross-reactivity or interference between DHT and analogues was observed.
