The DHX29 Polyclonal Antibody (CAB17709) is a powerful tool for researchers studying DHX29, a key RNA helicase involved in RNA metabolism and gene expression regulation. This rabbit-raised antibody is specifically designed for use in Western blot applications and is highly reactive with human samples. By binding to the DHX29 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for investigations in molecular biology and RNA biology.
DHX29, also known as DEAH box helicase 29, plays a crucial role in various cellular processes, including pre-mRNA splicing and ribosome biogenesis. Its importance in RNA metabolism makes it a valuable target for research on diseases related to defects in RNA processing, such as cancer and neurodegenerative disorders. Understanding the function of DHX29 is essential for developing potential therapeutic strategies that target RNA helicases in disease pathology.
Product Name:
DHX29 Rabbit Polyclonal Antibody
SKU:
CAB17709
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1020-1369 of human DHX29 (NP_061903.2).
This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells.
Purification Method:
Affinity purification
Gene ID:
54505
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using DHX29 Rabbit pAb (CAB17709) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
