The DiMethyl-eEF1A-K55 Polyclonal Antibody (CAB17987) is a high-quality antibody developed for reliable detection and analysis of target proteins. EEF1A is a highly conserved protein involved in protein synthesis and regulation. This antibody has been raised in rabbits and is highly reactive with human samples, making it ideal for use in Western blot applications.The Dimethyl-EEF1A (K55) Rabbit Polyclonal Antibody (CAB17987) specifically recognizes the dimethylated form of the EEF1A protein at lysine 55, allowing for precise detection and analysis in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DiMethyl-eEF1A-K55 Polyclonal Antibody
SKU:
CAB17987
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SFKY A
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
293T
Observed MW:
50kDa
Purification Method
Affinity purification
Gene ID
1915 1917
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using DiMethyl-eEF1A-K55 Rabbit pAb (CAB17987) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
