The DLDH/DLD Monoclonal Antibody (CAB5220) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using advanced rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting DLDH in various biological samples.DLDH plays a crucial role in the conversion of lactate to pyruvate, which is essential for ATP production and maintaining cellular functions. Dysregulation of DLDH activity has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative conditions. By targeting DLDH, researchers can gain insights into metabolic processes and develop new therapeutic strategies for treating these diseases.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DLDH/DLD Monoclonal Antibody
SKU:
CAB5220
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1224
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
SKOV3, U-251MG, Mouse liver, Mouse brain, Rat brain
Cellular Localization:
Mitochondrion Matrix, Nucleus.
Calculated MW:
54kDa
Observed MW:
56kDa
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1738
RRID
AB_2863492
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DLDH/DLD Rabbit mAb (CAB5220) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using DLDH/DLD Rabbit mAb (CAB5220) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using DLDH/DLD Rabbit mAb (CAB5220) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using DLDH/DLD Rabbit mAb (CAB5220) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using DLDH/DLD Rabbit mAb (CAB5220) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using DLDH/DLD Rabbit mAb (CAB5220) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using DLDH/DLD Rabbit mAb (CAB5220) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
