The DNA Ligase IV Antibody (CAB1743) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets DNA Ligase 4, an essential enzyme involved in the ligation of DNA strand breaks during DNA replication and repair processes.DNA Ligase 4 is a key player in the non-homologous end joining (NHEJ) pathway, which repairs double-strand breaks in DNA. Dysregulation of this pathway can lead to genomic instability, making DNA Ligase 4 a potential target for cancer therapy and genetic disease research. The CAB1743 antibody is highly reactive with human samples and has been validated for use in Western blot applications, enabling precise detection and analysis of DNA Ligase 4 protein expression in various cell types.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNA Ligase IV Antibody
SKU:
CAB1743
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LIG4S, DNA Ligase IV
Positive Sample:
PC-3
Cellular Localization:
Nucleus.
Calculated MW:
104kDa
Observed MW:
110kDa
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method
Affinity purification
Gene ID
3981
RRID
AB_2763789
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-3 cells, using DNA Ligase IV Rabbit pAb (CAB1743) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DNA Ligase IV Rabbit pAb (CAB1743) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
