The DNA Polymerase beta Monoclonal Antibody (CAB2412) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNA Polymerase beta Monoclonal Antibody
SKU:
CAB2412
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0752
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:200 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
POLB, DNA polymerase beta, DNA Polymerase beta
Positive Sample:
HeLa, A-431, Jurkat, Mouse testis, Mouse spleen, Mouse thymus, Rat testis, Rat spleen
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
38kDa
Observed MW:
42kDa
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
Purification Method
Affinity purification
Gene ID
5423
RRID
AB_2863003
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DNA Polymerase beta Rabbit mAb (CAB2412) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using DNA Polymerase beta Rabbit mAb (CAB2412) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
