The DNER Antibody (CAB15551) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is suitable for Western blot applications. By binding to the DNER protein, it allows for the accurate detection and analysis of DNER expression in a variety of cell types, making it ideal for neuroscience and developmental biology research.DNER, also known as Delta/Notch-like EGF-related receptor, plays a crucial role in mediating cell-to-cell communication and regulating neuronal activity.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNER Antibody
SKU:
CAB15551
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
bet, UNQ26, DNER
Positive Sample:
Hela, HT-29, Mouse brain, Mouse eye, Mouse small intestine, Rat brain
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
78kDa
Observed MW:
80kDa
Predicted to enable Notch binding activity. Involved in central nervous system development. Located in dendrite; early endosome; and plasma membrane.
Purification Method
Affinity purification
Gene ID
92737
RRID
AB_2762954
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNER Rabbit pAb (CAB15551) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of L929 cells using DNER Rabbit pAb (CAB15551) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
