The DNMT1 Antibody (CAB16729) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various experimental techniques including Western blotting.DNMT1 plays a crucial role in maintaining DNA methylation patterns during cell division and differentiation, and dysregulation of this enzyme has been implicated in various diseases including cancer and neurodevelopmental disorders. The DNMT1 Polyclonal Antibody enables researchers to detect and analyze DNMT1 expression levels in different cell types, allowing for a better understanding of its function in health and disease.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNMT1 Antibody
SKU:
CAB16729
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1786
RRID
AB_2769201
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and DNMT1 knockdown (KD) 293T cells using [KD Validated] DNMT1 Rabbit pAb (CAB16729) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 3 s.
Western blot analysis of lysates from Jurkat cells using [KD Validated] DNMT1 Rabbit pAb (CAB16729) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 3 s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KD Validated] DNMT1 Rabbit pAb (CAB16729) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
