Description
Anti-DNMT3A Antibody (CAB6503)
The DNMT3A Polyclonal Antibody (CAB6503) is a vital tool for researchers studying DNMT3A, a key enzyme involved in DNA methylation and gene regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the DNMT3A protein, researchers can detect and analyze its expression in various cell types, making it an essential tool for studies in epigenetics, cancer research, and developmental biology.DNMT3A plays a critical role in establishing and maintaining DNA methylation patterns, which are crucial for gene regulation and cellular differentiation.
Dysregulation of DNMT3A has been implicated in various diseases, including cancer, developmental disorders, and neurodegenerative diseases. Understanding the functions and mechanisms of DNMT3A is essential for developing targeted therapies and diagnostic tools for these conditions.Overall, the DNMT3A Polyclonal Antibody (CAB6503) provides researchers with a reliable and specific tool for investigating the role of DNMT3A in health and disease. Its high reactivity and specificity make it an ideal choice for researchers looking to expand their knowledge of epigenetic regulation and its implications in various pathological conditions.
| Antibody Name: | Anti-DNMT3A Antibody |
| Antibody SKU: | CAB6503 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human DNMT3A (NP_715640.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | MCF7, HT-29, BT-474, HepG2, SW620 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human DNMT3A (NP_715640.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SVTQ KHIQ EWGP FDLV IGGS PCND LSIV NPAR KGLY EGTG RLFF EFYR LLHD ARPK EGDD RPFF WLFE NVVA MGVS DKRD ISRF LESN PVMI DAKE VSAA HRAR YFWG NLPG MNRP LAST VNDK LELQ ECLE HGRI AKFS KVRT ITTR SNSI KQGK DQHF PVFM NEKE DILW CTEM ERVF GFPV HYTD VSNM SRLA RQRL L |
| Gene ID: | 1788 |
| Uniprot: | Q9Y6K1 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 16kDa/81kDa/101kDa |
| Observed MW: | 140kDa |
| Synonyms: | DNMT3A, DNMT3A2, M.HsaIIIA, TBRS, DNMT3A |
| Background: | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. |
| UniProt Protein Function: | DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. |
| UniProt Protein Details: | Protein type:EC 2.1.1.37; Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine; Methyltransferase Chromosomal Location of Human Ortholog: 2p23 Cellular Component: cytoplasm; euchromatin; nuclear matrix; nucleoplasm; nucleus Molecular Function:DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; identical protein binding; protein binding Biological Process: DNA methylation; genetic imprinting; negative regulation of gene expression, epigenetic Disease: Tatton-brown-rahman Syndrome |
| NCBI Summary: | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q9Y6K1 |
| NCBI GenInfo Identifier: | 166215081 |
| NCBI Gene ID: | 1788 |
| NCBI Accession: | Q9Y6K1.4 |
| UniProt Secondary Accession: | Q9Y6K1,Q86TE8, Q86XF5, Q8IZV0, Q8WXU9, E9PEB8, |
| UniProt Related Accession: | Q9Y6K1 |
| Molecular Weight: | 16,866 Da |
| NCBI Full Name: | DNA (cytosine-5)-methyltransferase 3A |
| NCBI Synonym Full Names: | DNA methyltransferase 3 alpha |
| NCBI Official Symbol: | DNMT3AÂ Â |
| NCBI Official Synonym Symbols: | TBRS; DNMT3A2; M.HsaIIIAÂ Â |
| NCBI Protein Information: | DNA (cytosine-5)-methyltransferase 3A |
| UniProt Protein Name: | DNA (cytosine-5)-methyltransferase 3A |
| UniProt Synonym Protein Names: | DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; M.HsaIIIA |
| Protein Family: | DNA |
| UniProt Gene Name: | DNMT3AÂ Â |
| UniProt Entry Name: | DNM3A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DNMT3A antibody (CAB6503) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of A549 cells using DNMT3A antibody (CAB6503). Blue: DAPI for nuclear staining. |
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