The DRAP1 Antibody (CAB17616) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to DRAP1 in human samples and has been validated for use in Western blot applications. By binding to DRAP1, this antibody enables precise detection and analysis of the protein in a variety of cell types, making it an ideal choice for studies in immunology and cancer research.DRAP1, also known as DNA damage-regulated autophagy modulator protein 1, plays a crucial role in maintaining cellular homeostasis by regulating autophagy, a process that removes damaged organelles and proteins to prevent cell damage and promote survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DRAP1 Antibody
SKU:
CAB17616
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NC2-alpha, DRAP1
Positive Sample:
U-87MG, Jurkat, 293T, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
22kDa
Observed MW:
32-38kDa
Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex.
Purification Method
Affinity purification
Gene ID
10589
RRID
AB_2769226
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DRAP1 Rabbit pAb (CAB17616) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
