The DSG1 Antibody (CAB9812) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, displays high specificity for human samples and is validated for use in various immunostaining applications. By targeting the Desmoglein-1 protein, this antibody allows for precise detection and analysis in different cell types, making it a valuable asset for studies in dermatology, oncology, and autoimmune diseases.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
DSG1 Antibody
SKU:
CAB9812
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Junction, Cell Membrane, Single-Pass Type I Membrane Protein, Desmosome.
Calculated MW:
114kDa
Observed MW:
150kDa
This gene encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of this gene has also been associated with the skin diseases palmoplantar keratoderma and erythroderma.
Purification Method
Affinity purification
Gene ID
1828
RRID
AB_2769230
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DSG1 Rabbit pAb (CAB9812) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of paraffin-embedded rat skin using DSG1 Rabbit pAb (CAB9812) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
