The DYNC1I1 Antibody (CAB3833) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables spectrin binding activity. Involved in vesicle transport along microtubule. Located in several cellular components, including kinetochore; recycling endosome; and spindle pole.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DYNC1I1 Antibody
SKU:
CAB3833
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:200 - 1:2000
IHC-P
1:20 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DNCI1, DNCIC1, DYNC1I1
Positive Sample:
Jurkat, SH-SY5Y, HeLa, Mouse testis, Mouse brain, Mouse skeletal muscle, Rat brain, Rat testis
Cellular Localization:
Chromosome, Cytoplasm, Centromere, Cytoskeleton, Kinetochore, Spindle Pole.
Calculated MW:
73kDa
Observed MW:
73kDa
Enables spectrin binding activity. Involved in vesicle transport along microtubule. Located in several cellular components, including kinetochore; recycling endosome; and spindle pole.
Purification Method
Affinity purification
Gene ID
1780
RRID
AB_2765333
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DYNC1I1 Rabbit pAb (CAB3833) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human stomach using DYNC1I1 Rabbit pAb (CAB3833) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
