The EDEM2 Antibody (CAB17181) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific for detecting EDEM2 in human samples and is validated for use in Western blot applications. By binding to the EDEM2 protein, this antibody allows for precise detection and analysis in various cell types, making it an essential asset for studies in cell biology and protein degradation mechanisms.EDEM2 is a key player in quality control processes within the ERAD pathway, where it helps identify and target misfolded proteins for degradation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
EDEM2 Antibody
SKU:
CAB17181
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C20orf31, C20orf49, bA4204.1, EDEM2
Positive Sample:
A375, HepG2
Cellular Localization:
Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Endoplasmic Reticulum Quality Control Compartment.
Calculated MW:
65kDa
Observed MW:
70kDa
In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).
Purification Method
Affinity purification
Gene ID
55741
RRID
AB_2769263
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EDEM2 Rabbit pAb (CAB17181) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunofluorescence analysis of L929 cells using EDEM2 Rabbit pAb (CAB17181) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
