The EIF2AK3 Antibody (CAB18196) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the EIF2AK3 protein, this antibody enables the detection and analysis of this important molecule in various cell types, making it ideal for studies in cell biology, immunology, and disease research.EIF2AK3, also known as PERK, is a critical mediator of the unfolded protein response, playing a key role in the cellular adaptation to stress conditions such as endoplasmic reticulum stress.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EIF2AK3 Antibody
SKU:
CAB18196
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PEK, WRS, PERK
Positive Sample:
Mouse thymus
Cellular Localization:
Endoplasmic Reticulum Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
125kDa
Observed MW:
140kDa
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
Purification Method
Affinity purification
Gene ID
9451
RRID
AB_2861973
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse thymus, using PERK Rabbit pAb (CAB18196) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using PERK Rabbit pAb (CAB18196) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using PERK Rabbit pAb (CAB18196) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using PERK Rabbit pAb (CAB18196) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
