The ELK1 Monoclonal Antibody (CAB19046) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is designed to detect Elk1 in human samples, making it an ideal choice for Western blot and immunohistochemistry applications. By targeting the Elk1 protein, researchers can gain insights into its function in various cellular processes, including gene expression regulation and cell signaling pathways.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ELK1 Monoclonal Antibody
SKU:
CAB19046
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0339
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ELK1
Positive Sample:
HeLa, K-562, Mouse brain, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
45kDa
Observed MW:
62kDa
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.
Purification Method
Affinity purification
Gene ID
2002
RRID
AB_2862539
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ELK1 Rabbit mAb (CAB19046) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg ELK1 antibody (CAB19046). Western blot was performed from the immunoprecipitate using ELK1 antibody (CAB19046) at a dilution of 1:1000.
