The eEF2 Antibody (CAB2068) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds to the Elongation Factor 2 protein, allowing for precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and cellular physiology.Elongation Factor 2 is a critical component of the protein synthesis machinery, facilitating the translocation of ribosomes along mRNA during translation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
eEF2 Antibody
SKU:
CAB2068
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EF2, EF-2, EEF-2, SCA26, eEF2
Positive Sample:
HeLa, NCI-H460, 293T
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
95kDa
Observed MW:
107kDa
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Purification Method
Affinity purification
Gene ID
1938
RRID
AB_2764091
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using eEF2 Rabbit pAb (CAB2068) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
