The Emerin/EMD Antibody (CAB1132) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced using rabbit immunization, is highly specific to human emerin and is validated for use in various applications, including Western blot and immunofluorescence.Emerin is involved in various cellular processes, including gene regulation, DNA repair, and cell signaling, making it a key player in nuclear organization and stability.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Emerin/EMD Antibody
SKU:
CAB1132
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Purification Method
Affinity purification
Gene ID
2010
RRID
AB_2758507
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Emerin/EMD Rabbit pAb (CAB1132) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human uterus using Emerin/EMD Rabbit pAb (CAB1132) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Confocal immunofluorescence analysis of U-2 OS cells using Emerin/EMD Rabbit pAb (CAB1132) at dilution of 1:100. Blue: DAPI for nuclear staining.
